Can Tylenol (acetaminophen) deplete glutathione levels in the body? Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol's Impact on Glutathione and Liver Damage Acetaminophen (Paracetamol) Springer Nature Link View of Acetaminophen, the missing anion gap in the patient with metabolic acidosis The Southwest journal of medicine The gamma glutamyl pathway after glutathione depletion due to Download Scientific Diagram