Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol depletes glutathione your liver's most important protective antioxidant. Every dose chips away at it. So instead of taking lower doses every 46 hours, I recommend spacing to 1000mg every 8 hours. Jenny McCarthy on Acetaminophen, Vaccines, and Autism How Glutathione is Depleted from the Body Suzy Cohen, RPh offers natural remedies to help you feel better now! Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Tylenol & Liver Health: Glutathione Boost