Glutathione depletion is reversible. fiercehealth.com Glutathione Depleting Pro Oxidant as a Selective Anticancer Therapeutic Agent ACS Omega Highly Selective and Prolonged Depletion of Mitochondrial Glutathione in Astrocytes Markedly Increases Sensitivity to Peroxynitrite Journal of Neuroscience How NSAIDs Affect Glutathione Levels Pure TheraPro Rx Glutathione depletion induces oxidative injury and apoptosis via TRPM2 channel activation in renal collecting duct cells ScienceDirect Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.