Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Illustration of the effects of acetaminophen on glutathione, Download Scientific Diagram Myth busting, Acetaminophen version. "Avoid Acetaminophen (Tylenol) because it causes glutathione depletion". Truth?? As a pediatrician and ER doctor and as moms we're often asked about this. And we understand why: The parent compounds of acetaminophen (US market in 1955 in the US as Tylenol and other products) were first discovered in 1886 (acetanilide) and 1887 (phenacetin). These were the earliest synthetic fever Acetaminophen Induced Hepatotoxicity: a Comprehensive Update The Hidden Impact of Acetaminophen Well Rooted