Do Vitamin B12 Supplements Cause Acne? VITAMIN B12 Supplements leading to ACNE BREAKOUTS?? Wellness Homeopathy Replying to @Angela Vitamin B12 supplements can trigger an acne like eruption, as can vitamin B6 & biotin supplements. In contrast to acne, there are no comedones. Once the supplements are stopped, Does B12 Cause Acne? (HINT: It Depends) Can Vitamin b12 Injections Cause Nasty Rashes? Is B12 making you break out? It's possible. We've found in our own experience and in clinical studies that certain forms of vitamin B12 can trigger inflammatory acne for some individuals.