Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Acetaminophen metabolism Tylenol And Glutathione Invigor Medical Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. In vivo upstream factors of mouse hepatotoxic mechanism with sustained hepatic glutathione depletion: Acetaminophen metabolite erythrocyte adducts and splenic macrophage generated reactive oxygen species ScienceDirect Acetaminophen Glutathione Disodium Salt CAS 64889 81 2 (free acid) SCBT Santa Cruz Biotechnology