GSH reduces NAPQI, the metabolite responsible for hepatotoxicity in acetaminophen toxicity. Toxic doses increase oxidation into NAPQI, overwhelming glutathione. How does ethanol interfere with acetaminophen metabolism? Image: https: t.co 6EY9dIwLXY NAPQI Wikipedia Two chromatographic methods for analyzing paracetamol in spiked human plasma with its toxic metabolite, N acetyl parabenzoquinone imine and its antidote, N acetyl l cysteine Scientific Reports NAPQI Wikipedia Liver injury induced by paracetamol and challenges associated with intentional and unintentional use Unveiling the molecular basis of paracetamol induced hepatotoxicity: Interaction of N acetyl p benzoquinone imine with mitochondrial succinate dehydrogenase ScienceDirect